BioFX® AP-Yellow One Component Microwell Substrate (pNPP)

Unique, patented, one-component pNPP colorimetric substrate for alkaline phosphatase microwell applications (Covered under U.S. Patent No. 7001717)

Product Code PNPS - BioFX® AP-Yellow One Component Microwell Substrate
Product Description

Contains p-nitrophenyl phosphate (pNPP)

Supplied as a one-component, ready-to-use solution

Unreacted substrate appears colorless to pale yellow

Soluble yellow product obtained upon reaction with phosphatase

Stop Reagent Stop the reaction using BioFX AP Stop Reagent (Product Code APSR)
Hazard Identification

Contains no aprotic solvents

Shelf Life

Stable for a minimum of 2 years from the date of manufacture when stored at 2°C - 8°C

Storage

Storage recommended at 2°C - 8°C

Substrate is light sensitive and should be protected from direct sunlight and UV sources

NOTES:

Not recommended for membrane or IHC applications that require a precipitating reaction product

Stabilizing pellets are available for redispensing purposes - please inquire

 

Signal-to-Noise Ratio Comparison

A comparison of a conventional pNPP one-component liquid to BioFX AP-Yellow One Component Microwell Substrate with stabilizing pellets was performed. Formulations were stored and tested at Days 0, 2, 4, 8, 15, and 30 under 37°C stress conditions. Results showed significantly higher S/N ratios for the BioFX substrate at every time point.

PNPS Graph

Product Brochure:  "BioFX® pNPP Colorimetric Substrate"