BioFX® Chemiluminescent AP Substrates

Five BioFX® chemiluminescent formulations are available for the detection of alkaline phosphatase (AP). These products offer ultra-high or routine detection based on dioxetane chemistry with detection potential to attogram sensitivity. These products are stable for a minimum of 24 months at 2°C to 8°C and are easy-to-use, one-component formulations.

Five BioFX Chemiluminescent options based on dioxetane chemistry

• AP Chemiluminescent Ultra Sensitive (450 nm) - Product Code APU4
• AP Chemiluminescent Super Sensitive (450 nm) - Product Code APS4
• AP Chemiluminescent Ultra Sensitive (540 nm) - Product Code APU5
• AP Chemiluminescent Super Sensitive (540 nm) - Product Code APS5
• AP Chemiluminescent Super Sensitive for Non-Reactive Media - Product Code APNR

Product Description

• Dioxetane-based chemistry
• Can detect alkaline phosphatase (AP) enzyme at attogram levels in a period of a few minutes
• 10 to 20 times more sensitive than typical dioxetane chemiluminescent reagents
• Designed for wavelengths with maximum emission at 450 nm or 540 nm
• Contain no organic solvents
• Provide low background with high signal-to-noise ratios
• Create a steady glow with a long-lasting light emission without signal decay for up to four hours
• Convenient, ready-to-use, one-component formulations
• Can be used in automatic or manual systems with a wide (5 to 6 log) dynamic range
• Detection platforms can include a wide variety of solid- and solution-phase applications

Ultra Sensitive vs. Super Sensitive Formulations

The two Ultra Sensitive formulations contain a signal enhancer that provides increased sensitivity. Customers are encouraged to test both formulations to determine which products work best in their specific assay.

Storage and Shelf Life

Stable for a minimum of 24 months at 2°C - 8°C when protected from light

 Product Brochure:  "SurModics Products for Immunoblotting"