In Vitro Diagnostic Products

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CHMI-0060-2CChemiluminescent Ultra Sensitive HRP Microwell Substrate - 60 mL (20 mL Luminol + 40 mL Buffer)

CHMI-0120-2CChemiluminescent Ultra Sensitive HRP Microwell Substrate - 120 mL (40 mL Luminol + 80 mL Buffer)

CHMI-0300-2CChemiluminescent Ultra Sensitive HRP Microwell Substrate - 300 mL (100 mL Luminol + 200 mL Buffer)

CHMM-0060-2CChemiluminescent Ultra Sensitive HRP Membrane Substrate - 60 mL (20 mL Luminol + 40 mL Buffer)

CHMM-0120-2CChemiluminescent Ultra Sensitive HRP Membrane Substrate - 120 mL (40 mL Luminol + 80 mL Buffer)

CHMM-0300-2CChemiluminescent Ultra Sensitive HRP Membrane Substrate - 300 mL (100 mL Luminol + 200 mL Buffer)

LUMI-0050-2CChemiluminescent Super Sensitive HRP Microwell and/or Membrane Substrate - 50 mL (25 mL Luminol + 25 mL Buffer)

LUMI-0110-2CChemiluminescent Super Sensitive HRP Microwell and/or Membrane Substrate - 110 mL (55 mL Luminol + 55 mL Buffer)

LUMI-0220-2CChemiluminescent Super Sensitive HRP Microwell and/or Membrane Substrate - 220 mL (110 mL Luminol + 110 mL Buffer)

LUMI-0440-2CChemiluminescent Super Sensitive HRP Microwell and/or Membrane Substrate - 440 mL (220 mL Luminol + 220 mL Buffer)

LUMI-0500-2CChemiluminescent Super Sensitive HRP Microwell and/or Membrane Substrate - 500 mL (250 mL Luminol + 250 mL Buffer)

LUMI-1000-2CChemiluminescent Super Sensitive HRP Microwell and/or Membrane Substrate - 1 L (500 mL Luminol + 500 mL Buffer)

LERI-0050-2CChemiluminescent Sensitive Plus HRP Microwell and/or Membrane Substrate - 50 mL (25 mL Luminol + 25 mL Buffer)

LERI-0110-2CChemiluminescent Sensitive Plus HRP Microwell and/or Membrane Substrate - 110 mL (55 mL Luminol + 55 mL Buffer)

LERI-0220-2CChemiluminescent Sensitive Plus HRP Microwell and/or Membrane Substrate - 220 mL (110 mL Luminol + 110 mL Buffer)

LERI-0440-2CChemiluminescent Sensitive Plus HRP Microwell and/or Membrane Substrate - 440 mL (220 mL Luminol + 220 mL Buffer)

LERI-0500-2CChemiluminescent Sensitive Plus HRP Microwell and/or Membrane Substrate - 500 mL (250 mL Luminol + 250 mL Buffer)

LERI-1000-2CChemiluminescent Sensitive Plus HRP Microwell and/or Membrane Substrate - 1 L (500 mL Luminol + 500 mL Buffer)

SERI-0050-2CChemiluminescent Sensitive HRP Microwell and/or Membrane Substrate - 50 mL (25 mL Luminol + 25 mL Buffer)

SERI-0110-2CChemiluminescent Sensitive HRPMicrowell and/or Membrane Substrate - 110 mL (55 mL Luminol + 55 mL Buffer)

SERI-0220-2CChemiluminescent Sensitive HRP Microwell and/or Membrane Substrate - 220 mL (110 mL Luminol + 110 mL Buffer)

SERI-0440-2CChemiluminescent Sensitive HRP Microwell and/or Membrane Substrate - 440 mL (220 mL Luminol + 220 mL Buffer)

SERI-0500-2CChemiluminescent Sensitive HRP Microwell and/or Membrane Substrate - 500 mL (250 mL Luminol + 250 mL Buffer)

SERI-1000-2CChemiluminescent Sensitive HRP Microwell and/or Membrane Substrate - 1 L (500 mL Luminol + 500 mL Buffer)

BioFX® Chemiluminescent HRP Substrates

Five chemiluminescent substrates with luminol-based formulations are available for HRP detection. These products offer ultra-high or routine detection with the potential for from picogram to femtogram sensitivity. These products are stable for a minimum of 18 months at 2°C to 8°C and are easy-to-use, two-component formulations.

Five chemiluminescent substrate options based on luminol chemistry

Product Description

Ultra-high to routine detection of HRP
Detection potential from picogram to femtogram sensitivity
Easy-to-use, two-component formulations
Available in both bulk and custom dispenses

Product Code	Chemiluminescent Ultra Sensitive HRP Microwell Substrate (Product Code CHMI)	Chemiluminescent Ultra Sensitive HRP Membrane Substrate (Product Code CHMM)	Chemiluminescent Super Sensitive HRP Microwell and/or Membrane Substrate (Product Code LUMI)	Chemiluminescent Sensitive Plus HRP Microwell and/or Membrane Substrate (Product Code LERI)	Chemiluminescent Sensitive HRP Microwell and/or Membrane Substrate (Product Code SERI)
Component Reagent A	Luminol (Product Code CHMA)	Luminol (Product Code CHMC)	Luminol (Product Code LUMA)	Luminol (Product Code LERA)	Luminol (Product Code SERA)
Component Reagent B	Reaction Buffer(Product Code CHMB)	Reaction Buffer(Product Code CHMD)	Reaction Buffer(Product Code LUMB)	Reaction Buffer (Product Code LERB)	Reaction Buffer (Product Code SERB)
Storage	Store at 2°C - 8°C protected from light in the original containers
Shelf Life	These chemiluminescent substrates are stable for a minimum of 18 months from the date of manufacture Mixtures of the two components (Reagents A and B) are stable for several hours at room temperature when protected from light
Product Use	Mix well and protect from light Mix 1 mL of Reagent A with 2 mL Reagent B Larger and smaller volumes of the substrate can be mixed at this same 1:2 ratio		Mix well and protect from light Mix 1 mL of Reagent A with 1 mL Reagent B Larger and smaller volumes of the substrate can be mixed at this same 1:1 ratio
NOTE	These chemiluminescent substrates are very sensitive and great care must be taken to optimize the individual assay components (e.g., antibodies, solid phase, etc.) to minimize background reactivity associated with nonspecific immunochemical reactions.
Detection Limit Comparison: BioFX HRP Microwell Substrate vs. Competitor	Rabbit anti-HRP was diluted and coated to the plate. Plate was sealed and incubated at 37°C for 1 hour then washed 3X with PBST. A diluted HRP standard curve was added to the plate. Standard curve ranged from 1000 pg/well to 15.625 pg/well. Plate was incubated for 1 hour at 37°C then washed 3X with PBST. Various substrates were added to appropriate wells. The plate was then incubated for 10 min. at R.T. prior to reading.
BioFX HRP Membrane Substrate	Mouse IgG was run on a 4%-20% reducing gel. Bands were transferred to a nitrocellulose membrane, blocked for 1 hour at 4°C, and incubated with HRP-conjugated rabbit anti-mouse IgG for 1 hour. After washing in PBST, BioFX chemiluminescent substrate (CHMM) was added and the blot visualized using a CCD camera. Note the low background and good contrast in the resulting image.

Product Brochure: BioFX^® HRP Chemiluminescent Substrates

Product Brochure: SurModics Products for Immunoblotting

Products on this Page:

Chemiluminescent Ultra Sensitive HRP Microwell Substrate

Chemiluminescent Ultra Sensitive HRP Membrane Substrate

Chemiluminescent Super Sensitive HRP Microwell and/or Membrane Substrate

Chemiluminescent Plus Sensitive HRP Microwell and/or Membrane Substrate

Chemiluminiscent Sensitive HRP Microwell and/or Membrane Substrate

Product Code	COA	Product Inserts	MSDS
CHMI	COA COA	Product Insert	MSDS MSDS
CHMM	COA COA	Product Insert	MSDS MSDS
LUMI	COA COA	Product Insert	MSDS MSDS
LERI	COA COA	Product Insert	MSDS MSDS
SERI	COA COA	Product Insert	MSDS MSDS

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BioFX® Chemiluminescent HRP Substrates

Five chemiluminescent substrate options based on luminol chemistry

Product Description

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