BioFX® DAB HRP Membrane Substrate

BioFX® DAB HRP Membrane Substrate increases stability and greatly improves sensitivity for rapid and simple detection of peroxidase in membrane applications. 

BioFX substrates are available in custom dispenses.  Contact us to discuss how we can meet your requirements.

BioFX DAB Membrane Substrate (Product Code DABM)
Product Description
  • Supplied as a two-component liquid substrate
  • DABC - Membrane Concentrate (Reagent A)
  • DABD - Membrane Reaction Buffer (Reagent B)
  • Reaction with peroxidase results in a light golden brown to dark brown precipitate
How it Works
  • DAB (3,3'-diaminobenzidine) is oxidized in the presence of peroxidase and hydrogen peroxide resulting in the deposition of a brown, alcohol-insoluble precipitate at the site of enzymatic activity
Shelf Life
  • Stable for a minimum of 3 years from the date of manufacture when stored at 2°C - 8°C in the original containers
  • After combination of the two reagents, the resulting 1X solution is stable for approximately 2 to 4 hours at room temperature (25°C)
  • The 1X solution may darken during this 2 - 4 hour time frame; this will not affect product performance
Storage
  • Store at 2°C - 8°C in the original container away from light and heat with lid tightly closed

Western Blot using DAB HRP Membrane Substrate – Comparisons were performed on western blots detecting serial dilutions of HSP 70 (10 to 0.04 μg) with anti-HSP-70 at a 1:2K dilution. Blots were washed 3X. A biotin-labeled Goat Anti-Mouse IgG was used as the secondary at 1:1K diltuion, Streptavidin-HRP was used at 1:1.5K with detection by BioFX DABM.

Find more out about SurModics BioFX substrates.

Product Brochure:  "SurModics Products for Immunoblotting"

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DAB Membrane Substrate