In Vitro Diagnostic Products
*Samples not available for some products.
Platinum Cured Silicone or C-flex tubing is recommended. One time use is highly recommended.
The Chemiluminescent AP Substrates can be used in automatic or manual systems with a wide dynamic range (5 to 6 log dynamic range).
Wash membrane with 0.2 M Tris buffer, pH 7.0 to 7.5 prior to substrate use.
Only use blocking reagents that are uncontaminated with residual alkaline phosphatase and toxin free.
BioFX Catalog number ABSS can be used to stop the reaction. 1% of sodium dodecyl sulfate can also be used.
Sample absorbance values should be monitored and read before absorbance values exceed 2.0 OD units.
To reduce or adjust the intensity of the reaction optimization of the antibody or conjugate is suggested.
Sample absorbance values should be monitored and read before absorbance values exceed 2.0 OD units.
Dilution of the substrates is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.
To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.
BioFX catalog numbers STPR, LSTP, BSTP, and LBSP are recommended. 1 N HCL and 0.6 N sulfuric acid can also be used.
A proprietary non-toxic, non-hazardous, non-carcinogenic solvent is used in the TMB Substrates
No.
To reduce or adjust the intensity of the reaction optimization of the antibody or conjugate is suggested.
TMBC is a system electrically controlled to generate a focused positive charge to a computer chip. Chelated metals in the TMBC make it possible to determine the conductivity and salinity of the substrate.
The conductivity of the TMBC is greater than or equal to 1500µS/cm.
The TMSK is approximately 25% less sensitive than the TMBW.
The TTMB is approximately 35% less sensitive than the TMBW.
The TMDS is approximately 60% less sensitive than the TMBW.
The TMBS is approximately 40% more sensitive than the TMBW.
E288.5 = 23,497 / E212 = 48,231 (c = 0.00447g/l in EtOH)
No. Most end users dissolve TMBP in organic solvents such as DMSO and DMF.
The removal of stabilizing pellets is not recommended.
The PNPS (pNPP substrate) can be read in the range of 405 nm to 420 nm.
BioFX catalog number APSR can be used. 50 µl of 3 N NaOH per 200 µl of PNPS can also be used.
The APBS is capable of being read in the range of 490 nm to 650 nm.
The mixed reagent must be used within 60 minutes.
BioFX catalog number APSR can be used to stop the APBS.
To stop the reaction, rinse the membrane with reagent quality water.
The APRS is capable of being read in the range of 450 nm to 550 nm.
Following the combination of the two components, the resulting 1X solution is stable for 2 - 4 hours at 25°C and up to 8 hours at 2°C to 8°C.
Stop the reaction by gently rinsing the stained membrane in 2 - 3 changes of distilled water.
To stop the reaction, rinse the membrane with reagent quality water.
Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.
The presence of flocculent material will not affect product performance.
Use substrate immediately after combining the components.
The solution is stable for up to 1 hour. Some color and turbidity may develop over this period that does not affect product performance.
To stop the reaction, rinse the membrane with reagent quality water.
These products are not recommended for immunohistochemical applications.
The EoProbe signal can be seen with both brightfield and under fluorescence.
Fluorescence should be performed with an excitation filter of 510 - 590 nm, dichroic mirror of 580 nm and a barrier filter of 590 nm.
The EOPB is not recommended for flow cytometry applications even though automated cyto-imaging systems can be used.
BioFX catalog number FLPM is recommended for mounting.
Continuous photoexcitation should be used for at least a minute in order to photobleach all nonspecific autofluorescence.
Following the combination of the two components, the resulting 1X solution is stable for 2 - 4 hours at 25°C and up to 8 hours at 2°C to 8°C.
Stop the reaction by gently rinsing the stained membrane in 2 - 3 changes of distilled water.
Counterstain the section with hematoxylin or methyl green.
The solution is stable for up to 1 hour but some color and turbidity may develop over this period that does not affect product performance.
Both hematoxylin and methyl green are appropriate counterstains.
Dehydrate section in alcohol or another organic solvent and clear in xylene.
No.
After target specimen is covered with FLPM and a coverslip, evaluate specimen as soon as possible.
The APHP blocks both alkaline phosphatase and peroxidase endogenous enzymatic activity.
The MLKB can serve as a diluent for antigens and antibodies when diluted in a 4% solution of glycerol.
As a blocking reagent, the solution binds to the secondary protein binding sites following the binding of the primary antigen or antibody to the solid phase surface.
To avoid complex formation with ionic species such as calcium and magnesium in blood, Tris buffers are preferable over phosphate buffers.
When using 1X solution for coating plates, best results are obtained following an overnight incubation at 2°C - 8°C.
The optimal pH range for diluted TBSC is 7.4 - 7.8 at 22°C to 28°C.
PBSC should not be used in assays where there is a competition for phosphate groups, if complex formulations with metal ions are essential for the enzyme activation.
When using 1X solution for coating plates, best results are obtained following an overnight incubation at 2°C - 8°C.
The optimal pH range for diluted PBSC is 7.2 - 7.6 at 22°C to 28°C.
Phosphate ions will inhibit carboxypeptidiase, carboxylas, urease, muscle diaminase, formase, and phosphoglucomutase.
The solutions contain 0.5% Tween 20, which has no net charge to interfere with assay reactants.
STPR reacts more slowly, is less volatile and does not form corrosive hydrogen chloride.
At least one hour.
Classification III - mildly corrosive with a time of 55 minutes.
A proprietary Amphipathetic blend containing alpha and beta unsaturated Carbonyl compound.
Prior to hydration with water, the STPR is stable indefinitely. Following hydration, the reagent is stable for a minimum of 3 years from the date of reconstitution when stored at 25°C.
Classification III - mildly corrosive with a time of 55 minutes
At least one hour.
A proprietary Amphipathetic blend containing alpha and beta unsaturated Carbonyl compound.
The BSTP is a proprietary dry blend.
Prior to hydration with water, the BSTP is stable indefinitely. Following hydration, the reagent is stable for a minimum of 3 years from the date of reconstitution when stored at 25°C.
Refrigerated temperatures will not harm the reagent.
Refrigerated temperatures will not harm the reagent.
The pH of the LBSP is 7.5 - 11.5.
The ABSS is a proprietary dry blend.
Prior to hydration with water, the ABSS is stable indefinitely. Following hydration, the reagent is stable for a minimum of 3 years from the date of reconstitution when stored at 25°C.
The reagent is stable for 3 years following hydration.
Use 50 µl of AP Stop Reagent per 200 µl of APRS and APBS, use equal volumes of APSR and PNPS.
Prior to hydration with water, the APSR is stable indefinitely. Following hydration, the reagent is stable for a minimum of 3 years from the date of reconstitution when stored at 25°C.
Samples should be blocked for a minimum of 10 minutes at 25°C.
Incubate a minimum of 3 hours.
Antimicrobial agents have been added to retard bacterial growth and are not considered corrosive at the concentration supplied.
Samples should be blocked for a minimum of 10 minutes at room temperature. Additional blocking time may be required depending on tissue thickness and sample penetrability.
Yes.
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