StabilCoat® Plus Microarray Stabilizer

Use StabilCoat® Plus Microarray Stabilizer to improve the stability of proteins covalently bound to microspheres stored in solution and array proteins stored in the dried state.

  • StabilCoat Plus stabilizer not only protects the activity of proteins, but has the additional and rare ability to block nonspecific binding sites on microspheres.
  • StabilCoat Plus solution also prevents microsphere aggregation, keeping them monodispersed even after long-term storage. Note: StabilCoat Plus stabilizer should not be used with proteins that are non-covalently adsorbed to microspheres.
  • The conformation and activity of proteins on microarrays are also effectively preserved in the dried state when stored long-term with StabilCoat Plus stabilizer.
  • Produce more accurate results on array slides by increasing signal, reducing background, and improving sensitivity. Varying results may be expected among different protein array surface chemistries.
Product Number

SC02 - StabilCoat® Plus Microarray Stabilizer
Available Sizes

50 mL sample, 1000 mL
Storage

Product should be refrigerated or stored at room temperature.
Bovine Protein

Yes
Dry Weight (g/mL)

0.098 - 0.113
Product Buffer

PBS
pH

7.0 - 7.4
Preservative

0.02% methylisothiazolone and 0.02% bromonitrodioxane
Shelf Life

3 years
Stability Data - Microspheres

Accelerated stability studies were conducted to evaluate the effectiveness of StabilCoat Plus stabilizer to block and stabilize rabbit polyclonal IgG antibody covalently attached to polystyrene microspheres in solution. Antibody-coated microspheres were stored in solution at 45°C with either StabilCoat Plus stabilizer or a control buffer. StabilCoat Plus stabilizer retained >96% binding activity of the antibody coated to the microspheres after 79 days at 45°C.
Stability Data - Microarrays

Monoclonal antibody to IFNg (100 and 300 µg/mL) was arrayed on a SurModics protein-binding surface using BioRobotics split pins and arrayer. Cocktail antigens containing recombinant human IFNg (25 ng/mL) were incubated on slides using either a control buffer or StabilCoat Plus stabilizer. Arrays were developed with biotinylated antibody cocktail suspended in either the control buffer or StabilCoat Plus stabilizer.The spots were visualized using Streptavidin Cy5®. Enhanced signal intensity was achieved using StabilCoat Plus stabilizer.

 Product Brochure:  "SurModics Microarray Products"

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StabilCoat® Plus Microarray Stabilizer