StabilGuard® Choice Microarray Stabilizer (BSA-Free)

Improve the stability of proteins bound to microspheres stored in solution and array proteins stored in the dried state, without the use of BSA protein, using StabilGuard® Choice Microarray Stabilizer (BSA-Free).

  • StabilGuard Choice stabilizer protects the activity of proteins while simultaneously blocking nonspecific binding sites on microspheres. It also prevents microsphere aggregation, keeping them monodispersed even after long-term storage. Note: StabilGuard Choice stabilizer should not be used with proteins adsorbed to microspheres.
  • Conformation and activity of proteins on arrays are effectively preserved long-term in a dried state when stored with StabilGuard Choice stabilizer. Results are produced on array slides by increasing signal, reducing background, and improving sensitivity.
Product Number

SG02 - StabilGuard® Choice Microarray Stabilizer (BSA-Free)

Available Sizes

50 mL sample, 1000 mL

Storage

Product should be refrigerated or stored at room temperature.

Bovine Protein

No

Dry Weight (g/mL)

0.057 - 0.066

Product Buffer

PBS

pH

6.6 - 7.2

Preservative

0.02% methylisothiazolone and 0.02% bromonitrodioxane

Shelf Life

3 years

Stability Data - Microspheres

Accelerated stability studies were conducted to evaluate the effectiveness of StabilGuard Choice stabilizer to block and stabilize rabbit polyclonal IgG antibody covalently attached to polystyrene microspheres in solution. Antibody-coated microspheres were stored in solution at 45°C with either StabilGuard Choice stabilizer or a control buffer. StabilGuard Choice stabilizer retained >96% binding activity of the antibody coated to the microspheres after 79 days at 45°C.

Stability Data - Microarrays

Monoclonal antibody to IFNg (100 and 300 µg/mL) was arrayed on a SurModics protein-binding surface using BioRobotics split pins and arrayer. Cocktail antigens containing recombinant human IFNg (25 ng/mL) were incubated on slides using either a control buffer or StabilGuard Choice stabilizer. Arrays were developed with biotinylated antibody cocktail suspended in either the control buffer or StabilGuard Choice stabilizer. The spots were visualized using Streptavidin Cy5. Enhanced signal intensity was achieved using StabilGuard Choice stabilizer.

 Product Brochure:  "SurModics Microarray Products"