Frequently Asked Questions - StabilZyme Stabilizers

What is the difference between StabilZyme HRP, StabilZyme SELECT and StabilZyme NOBLE stabilizers?

StabilZyme HRP contains components that increase enzyme activity. While this is beneficial in some assays, it causes interference in others, such as competitive assays.

StabilZyme SELECT was developed to provide stabilization comparable to that of StabilZyme HRP, but without increasing enzyme activity that can interfere with the standard curve in competitive assays. StabilZyme SELECT is also recommended for direct or sandwich assays where background is a problem.

StabilZyme NOBLE is a BSA-free alternative to StabilZyme SELECT.  It is designed to be used for conjugated proteins, antibody-coated particles, and unmodified proteins in solution.

What is the shelf life of the StabilZyme Stabilizers when stored at 4°C?

All StabilZyme stabilizers have a shelf life of 30 months when stored at 4°C. Testing was performed on StabilZyme stabilizers with no conjugate added. Products were stored at 37ºC and 4ºC, and then tested for retained activity after multiple time points up to 30 months. Studies were based on a goat anti-rabbit IgG antibody.

What shelf life can I expect from a conjugate added to the StabilZyme Stabilizers?

Real-time studies indicate that a shelf life of 24 months at room temperature is achievable.

What can be used to dilute the StabilZyme Stabilizers?

We recommend using the stabilizers undiluted for maximum stabilization.  However, in cases where you feel it may be necessary to dilute the products (e.g., 1:1) please use the following diluents:

--StabilZyme HRP Stabilizer can be diluted in deionized water.

--StabilZyme AP Stabilizer can be diluted in deionized water or 10mM MES buffer, pH 6.0.

--StabilZyme SELECT Stabilizer can be diluted in deionized water or 0.1 M Tris or Glycine buffer.

--StabilZyme NOBLE Stabilizer can be diluted in deionized water.

NOTE: Phosphate buffers cause precipitation in the StabilZyme products when used at >10% total volume.

Can StabilZyme HRP stabilize the horseradish peroxidase enzyme by itself?

Yes.

What should I do if there is an increased signal when I use StabilZyme HRP Stabilizer in my assay?

In some cases, StabilZyme HRP stabilizer increases the signal of the conjugate. If this happens, we recommend trying StabilZyme SELECT, or StabilZyme NOBLE Stabilizer.

What dilution of antibody conjugate is used for the Quality Control testing for StabilZyme AP Stabilizer?

Our standard ELISA uses a 1:3000 dilution of the conjugate in StabilZyme AP.  We recommend diluting your AP conjugate at your normal use concentration first. You may consider trying a small accelerated study to determine whether or not you are getting the stability you need. In our experience, we have found that there can be a large difference in AP conjugate quality and stability depending upon the supplier.

What dilution of antibody conjugate is used for the Quality Control testing for StabilZyme HRP, SELECT, and NOBLE Stabilizers?

Our standard ELISA uses a 1:10,000 dilution of the conjugate in StabilZyme HRP, SELECT, and NOBLEstabilizers.  We recommend you first try diluting the HRP conjugate to your normal use concentration. You may consider a small accelerated study to determine whether or not you are getting the stability you need.

Can I freeze-dry proteins with StabilZyme stabilizers?

Freeze-drying proteins with the StabilZyme products is not recommended.

Can the StabilZyme stabilizers be used in serum controls?

Yes. We recommend StabilZyme SELECT and NOBLE stabilizers for stabilizing serum controls.

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