In Vitro Diagnostics Products & Technologies

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StabilCoat® Plus

Microarray Stabilizer


StabilCoat Plus Microarray Stabilizer improves the stability of proteins bound to microspheres stored in solution, and array proteins stored in the dried state.

Product Number

SC02

Available Sizes

50 mL sample, 1000 mL

Storage

Product should be refrigerated or stored at room temparature.

Bovine Protein

Yes

Dry Weight (g/mL)

0.098 - 0.113

Product Buffer

PBS

pH

7.0 - 7.4

Preservative

0.02% methylisothiazolone, 0.02% bromonitrodioxane

Shelf Life

3 years

Acceptable Diluents

Deionized water or PBS

Stability Data - Microspheres

Accelerated stability studies were conducted to evaluate the effectiveness of StabilCoat Plus stabilizer to block and stabilize rabbit polyclonal IgG antibody covalently attached to polystyrene microspheres in solution.  Antibody-coated microspheres were stored in solution at 45ºC with either StabilCoat Plus stabilizer or a control buffer.  StabilCoat Plus stabilizer retained >96% binding activity of the antibody coated to the microspheres after 79 days at 45ºC.

Stability Data - Microarrays

Monoclonal antibody to IFNg  (100 and 300 µg/mL) was arrayed on a SurModics protein-binding surface using BioRobotics split pins and arrayer.  Cocktail antigens containing recombinant human IFNg (25 ng/mL) were incubated on slides using either a control buffer or StabilCoat Plus stabilizer.  Arrays were developed with biotinylated antibody cocktail suspended in either the control buffer or StabilCoat Plus stabilizer.  The spots were visualized using Streptavidin Cy5.  Enhanced signal intensity was achieved using StabilCoat Plus stabilizer.